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MBG 515E - Purification Techniques for Biomolecules

Course Description

Overview of bioseparations: The nature of the problems, Specification of a general strategy for separation and purification; Removal of insolubles: Filtration techniques, Centrifugation techniques, Broth conditioning; Product isolation: Cell distruption, Extraction, Product enrichment; Purification of the product: Principles of chromatographic separations, Elution chromatography, Ion-exchange chromatography, Gel permeation chromatography, Hydrophobic interactions chromatography, Adsorption chromatography, Affinity chromatography; High Pressure Liquid Chromatography: Theoritical Aspects, Applications; Ultrafiltration; Electrophoresis; Analysis of protein purity; Polishing as a final purification step: Crystallization, Drying.

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