BIO 328EL - Biochemistry II Lab

Course Objectives

1. What are the parameters to growth the cell having industrially important recombinant enzyme
2. What are the basic steps to purify the recombinant protein
3. Characterisation of the purified recombinant protein.

Course Description

Industrially important recombinant enzyme is produced. Following by the purification of this enzyme by using different types of chromatography techniques, characterisation is carried out by using the gel electrophoresis and enzyme kinetic experiments.