Course Information

Course Name	Turkish	Biyokimya II Laboratuar
	English	Biochemistry II Lab
Course Code	BIO 328EL	Credit	Lecture (hour/week)	Recitation (hour/week)	Laboratory (hour/week)
Semester	5	5	-	-	3
Course Language		English
Course Coordinator		Nevin Gül Karagüler
Course Objectives		1. What are the parameters to growth the cell having industrially important recombinant enzyme 2. What are the basic steps to purify the recombinant protein 3. Characterisation of the purified recombinant protein.
Course Description		Industrially important recombinant enzyme is produced. Following by the purification of this enzyme by using different types of chromatography techniques, characterisation is carried out by using the gel electrophoresis and enzyme kinetic experiments.
Course Outcomes		Students who pass the course will be able to: I. Preparation of solution and media II. Cell growth and enzyme expression III. Physical and enzymatic cell disruption methods IV. Protein precipitation depends on physical and chemical properties of the protein V. Removing methods of the undesirable small molecules VI. Application of purification of proteins by chromatographic methods VII. Understanding of enzyme-substrate interaction and interpretation of kinetic data
Pre-requisite(s)		BIO 315EL MIN CC or BIO 311E MIN DD
Required Facilities
Other
Textbook
Other References