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Course Weekly Lecture Plan

Week Topic
1 Introduction to Microbial Ecology of Natural and Engineered Ecosystems:
-Importance of studying microbial ecology
-Advantages of molecular tools over traditional tools
-Target molecules
2 Basic Molecular Tools:
DNA and RNA isolation; quan. and qual. analyses of nucleic acids (spec, agarose gel electroph., PAGE); PCR; reverse transcript.
3 Assessment of Microbial Diversity-I:
Cloning and Sequencing
4 Assessment of Microbial Diversity-II:
DGGE; TRFLP (ARDRA)
5 Quantification of Certain Microbial Groups-I:
Real time PCR
6 Quantification of Certain Microbial Groups-II:
FISH
7 Assessment of Function of Microbial Groups-I:
Proteomics,
RNA-targeted analyses
8 Assessment of Function of Microbial Groups-II:
Microarray; FISH-MAR; SIP
9 Submission of Term-Papers (Written Reports)
10 Student Presentation-1:
Cloning and sequencing
DGGE
11 Student Presentation-2:
Real Time PCR (Q-PCR)
FISH
12 Student Presentation-3:
Microarray
SIP
13 Student Presentation-4:
Proteomics
Phospholipid fatty acid (PLFA) analysis
14 Overall Assessment
 
 
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