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ENB 602E
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Course Weekly Lecture Plan
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Course Information
Course Weekly Lecture Plan
Course Evaluation Criteria
Course Weekly Lecture Plan
Week
Topic
1
Introduction to Microbial Ecology of Natural and Engineered Ecosystems:
-Importance of studying microbial ecology
-Advantages of molecular tools over traditional tools
-Target molecules
2
Basic Molecular Tools:
DNA and RNA isolation; quan. and qual. analyses of nucleic acids (spec, agarose gel electroph., PAGE); PCR; reverse transcript.
3
Assessment of Microbial Diversity-I:
Cloning and Sequencing
4
Assessment of Microbial Diversity-II:
DGGE; TRFLP (ARDRA)
5
Quantification of Certain Microbial Groups-I:
Real time PCR
6
Quantification of Certain Microbial Groups-II:
FISH
7
Assessment of Function of Microbial Groups-I:
Proteomics,
RNA-targeted analyses
8
Assessment of Function of Microbial Groups-II:
Microarray; FISH-MAR; SIP
9
Submission of Term-Papers (Written Reports)
10
Student Presentation-1:
Cloning and sequencing
DGGE
11
Student Presentation-2:
Real Time PCR (Q-PCR)
FISH
12
Student Presentation-3:
Microarray
SIP
13
Student Presentation-4:
Proteomics
Phospholipid fatty acid (PLFA) analysis
14
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